Prediction of the Trace Amounts of Atomoxetine in Biological Samples using optimized solvent bar microextraction technique coupled with HPLC-UV
Authors
Abstract:
Atomoxetine, an attention-deficit/hyperactivity disorder (ADHD) agent , has severe adverse effects, which even causes suicidal ideation in patients and when combined with other drugs such as monoamine oxidase inhibitors or in patients with cardiovascular disorders or pheochromocytoma, it can be fatal. In order to monitor the trough level of the drug, sensitive analytical methods are in demand.Therefore, for detection of trace levels of this drug, the trend solvent bar microextraction method coupled with HPLC-UV were implemented. Application of pH gradient of 10.3 and 3.0 (donor and acceptor phase, respectively) enabled preconcentration and microextraction of the target analyte. The results were modeled using Artificial Neural Network (ANN) successfully.The optimum results were obtained with a stirring rate of 365 rpm and salt addition by 15.1% at 25°C after 26 min. The limit of detection and quantification were 7.0 ng mL-1and 20 ng mL-1, respectively. It offered good linearity range of 20-5000 ng mL-1 with a coefficient of estimation higher than 0.9972 and a preconcentration factor of 112. The relative standard deviations of analysis were 4.6% within a day (n=3) and 6.2% between days (n=9).Overall, this method can be employed for several purposes such as for dosage adjustment in ADHD patients, clean validation in the pharmaceutical industry, and for trace determination in forensics.
similar resources
Microextraction and Determination of Diclofenac in Biological Samples using Hollow Fiber Liquid Phase Microextraction Technique Coupled with HPLC-UV
In this study, hollow fiber liquid phase microextraction (HF-LPME) coupled with highperformanceliquid chromatography (HPLC) with UV detection was applied for preconcentrationand determination of Diclofenac sodium in biological fluids. Parameters affecting the extractionprocess including pH of donor phase and acceptor phase, type of extraction solvent, stirring rate,extraction time, and salt add...
full textOptimized preconcentration of Imatinib using dispersive liquid-liquid microextraction coupled with HPLC-UV
Background: Imatinib is an antineoplastic agent acting as a tyrosine kinase inhibitor to treat cancer such as lymphoblastic leukemia. The dosage of the anticancer drugs plays a critical role in the survival of the patients. For this reason, the patient's plasma and urine samples should be monitored to obtain the necessary information regarding the toxicity of the drug. In this study, the applic...
full textTrace determination of gatifloxacin in pharmaceutical and biological samples using hydrophobic magnetic nanoparticles-assisted ionic liquid microextraction coupled with spectrofluorimetry
A simple and rapid microextraction procedure based on the application of magnetic nanoparticles and hydrophobic 1-Hexylpyridinium hexafluorophosphate [Hpy][PF6] ionic liquid (IL) was applied to trace determination of gatifloxacin. A mixture of [Hpy][PF6] and magnetic nanoparticles was injected into the sample solution containing the analyse of interest and a cloudy solution was forme...
full textTrace determination of gatifloxacin in pharmaceutical and biological samples using hydrophobic magnetic nanoparticles-assisted ionic liquid microextraction coupled with spectrofluorimetry
A simple and rapid microextraction procedure based on the application of magnetic nanoparticles and hydrophobic 1-Hexylpyridinium hexafluorophosphate [Hpy][PF6] ionic liquid (IL) was applied to trace determination of gatifloxacin. A mixture of [Hpy][PF6] and magnetic nanoparticles was injected into the sample solution containing the analyse of interest and a cloudy solution was forme...
full texttrace determination of gatifloxacin in pharmaceutical and biological samples using hydrophobic magnetic nanoparticles-assisted ionic liquid microextraction coupled with spectrofluorimetry
a simple and rapid microextraction procedure based on the application of magnetic nanoparticles and hydrophobic 1-hexylpyridinium hexafluorophosphate [hpy][pf6] ionic liquid (il) was applied to trace determination of gatifloxacin. a mixture of [hpy][pf6] and magnetic nanoparticles was injected into the sample solution containing the analyse of interest and a cloudy solution was formed. after ce...
full textthe trace of translators ideology in literary translations: a case study of persian translation of the scaret letter
رساله ی حاضر تلاشی است برای بررسی مبحث ایدئولوژی در ترجمه ادبی و تاثیرات آن بر کار مترجم، اعم از گزینش واژگان و ساختارهای دستوری؛ با تمرکز بر تحلیل انتقادی گفتمان در متن مبدأ و متن مقصد، که در این رساله متن مبدأ رمان داغ ننگ اثر ناتانیل هاوثورن و ترجمه فارسی سیمین دانشور به عنوان متن مقصد مورد بررسی قرار گرفته است. تجزیه و تحلیل رمان های مورد نظر به طور عمده بر اساس مدل پیشنهادی فرحزاد(2007) بر...
My Resources
Journal title
volume 13 issue 4
pages 28- 41
publication date 2019-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023